the extent to which estradiol, progesterone, and testosterone influence mating behavior across the menstrual cycle is unclear. the proactive and responsive mating strategies scales (parmss) were developed to separately examine two specific components of sexuality and were used here to test divergent hormonal associations. preliminary psychometric data (n = 364 females) suggest that both scales consist of one factor and demonstrate strong psychometric properties (internal consistency, test–retest reliability, and construct and convergent validity). the parmss were used in a repeated-measures observational study to examine the relationships between changes in endogenous hormone levels and both proactive and responsive mating intentions with potential new short-term or long-term partners in healthy pre-menopausal participants (n = 38). at two points in their cycle, participants provided salivary hormone samples in the laboratory and reported the likelihood of engaging in proactive and responsive behaviors with men in photos and vignettes. participants reported greater responsive than proactive intentions. increases in estradiol and testosterone across the cycle were associated with increases in short-term mating intentions, particularly responsivity to potential short-term relationship partners. no associations were found for intentions that were proactive or that involved potential long-term partners or for progesterone. changes in the three hormones explained changes in short-term responsive mating intentions (22% of the variance). the results suggest (a) cyclical changes in estradiol and testosterone are differentially associated with changes in responsive vs. proactive mating intentions and (b) context-dependent changes (i.e., short-term vs. long-term mating intentions and possibly relationship status). the findings require replication with larger and diverse samples.